(Solution) SCIE1046 Activity 2.2 Aseptic Technique Culture your sample without contamination

COURSE  

SCIE1046: Fundamental of Microbiology with Lab

1. About the Lab

Learning Objectives:

  • Understand the principles of aseptic technique for the prevention of infection and contamination.
  • Create and maintain a sterile work area.
  • Use sterile equipment and consumables correctly.
  • State potential sources of microbial contamination.
  • Assess whether a sample was contaminated.

Estimated Length: 15 minutes

 

MAKE THE CONNECTION

The background information in section 2 was adapted from the following Microbiology lecture course Tutorials:
4.1.1: Bacterial Growth
4.1.4: Media Used for Microbial Growth

 

2. Background Information

The following background information will be helpful as you prepare for the simulation.

2a. Nutrition Requirements of Media Used for Microbial Growth

Growth medium needs to contain sufficient nutrients and vitamins to support the growth of the microbes of interest. Many growth media are considered general all-purpose media because they are intended to support a wide range of microorganisms. Commonly used options are tryptic soy broth (as a liquid medium) and tryptic soy agar (as a solid medium).

In some cases, specialized media are needed. These media may be used to grow bacteria with specialized requirements or for particular purposes.

Differential media are useful in distinguishing between types of bacteria because of changes in the bacteria or medium.

 

TERMS TO KNOW

These glossary terms are important to know and will help you during the Activity.

Growth Media

Media that contain a variety of nutrients necessary to sustain the growth of microorganisms. Also called growth medium.

Differential Media

Media that are used to distinguish between types of bacteria because of changes in the bacteria or medium. Also called differential medium.

Specialized Media

Media that inhibit the growth of unwanted microorganisms and supports the growth of the organism of interest. Also called selective medium.

 

2b. Bacterial Growth

You are encouraged to review the complete Microbiology lecture course Tutorial 4.1.1: Bacterial Growth for additional background on this topic before you begin the simulation.

3. Lab Manual

  Lab Manual – Aseptic Technique: Culture your sample without contamination

This Lab Manual gives a synopsis of the lab and the theory behind it. You’re encouraged to read or download the manual before launching the lab. This information will also be available during the simulation by selecting the “Theory” tab on the virtual LabPad.

4. Launch Lab

You’re ready to begin! Review the helpful navigation tips below. Then click the Launch Lab button to start your lab. Be sure to answer all the questions in the simulation because they contribute to your score. Good luck, scientists!

  • Exiting:To exit a lab simulation, press the ESC key on your keyboard. This key returns you to the objective screen for the simulation.
  • Saving:You do not need to complete a simulation in one sitting. Labster saves your progress at predetermined checkpoints upon exit. To see your progress at any time, click on the mission tab of the LabPad.
  • Restarting:You are allowed an unlimited number of restarts for a simulation to improve your quiz score. Sophia and Labster will always store your best score.
  • Just Browsing:You can restart a simulation to have a look around without completing it. The program will still retain your previous (and best) score.

 

HINT

Visit Labster’s Help Center for technical information and support. (Double check that the quotes surrounding the URL are straight rather than curly after copying into Sophia; retype them if needed.)