COURSE   SCIE1046: Fundamental of Microbiology with Lab About the Lab Learning Objectives: Explain how and why microbial colonization occurs. Recognize potential sources of contamination. Describe the consequences of unregulated population growth. Describe the ideal environments for microbial growth and how they can be manipulated. Appreciate different levels of selective toxicity. Describe modes of microorganism growth control. Define selective toxicity and w...

COURSE   SCIE1046: Fundamental of Microbiology with Lab 1. About the Lab Learning Objectives: Understand the concept of food spoilage and shelf life. Understand the principle of pasteurization and sterilization. Analyze the parameters of High Temperature Short Time (HTST) pasteurization. Perform canning as a method of sterilization. Understand how plastic and metal can be used as materials for packaging. Estimated Length: 40 to 45 minutes   MAKE THE CO...

COURSE   SCIE1046: Fundamental of Microbiology with Lab Activity 5.4: Biosafety 1. About the Lab Learning Objectives: Understand how a Biosafety containment level 3 laboratory is constructed (e.g. air flow and pressure control). Understand the basic safety rules of a Biosafety containment level III laboratory (e.g. use of safety equipment). Handle microorganisms in a Biosafety containment level III laboratory. Understand the concept of fumigation and how this is execu...

1. About the Lab Learning Objectives: Distinguish vertical gene transfer from horizontal gene transfer. Understand the concept of genetic variability and survivability in bacteria. Describe the concept of horizontal gene transfer. Identify genetic elements and cell machinery required for DNA transfer. Outline the main events that occur during conjugation, transformation, and transduction. Discuss the outcome and barrier of genetic transfer in bacteria. Estimated Length: 3...

COURSE   SCIE1046: Fundamental of Microbiology with Lab . About the Lab Learning Objectives: Explain the principle of different ELISA techniques. Apply sandwich ELISA to quantify protein samples. Analyze the standard curve of ELISA experiment. Understand the function of reagents and equipment used in ELISA. Describe the basic troubleshooting process of ELISA. Estimated Length: 50 minutes to 1 hour   MAKE THE CONNECTION The background information in ...

COURSE   SCIE1046: Fundamental of Microbiology with Lab About the Lab Learning Objectives: Build a transfer stack with the correct orientation. Set up a transfer tank to transfer protein from an SDS-PAGE gel to a membrane. Critique the quality of protein transfer using a removable protein-identifying stain. Estimated Length: 35 to 45 minutes   MAKE THE CONNECTION The background information in section 2 was adapted from the following Microbiology lecture c...

COURSE   SCIE1046: Fundamental of Microbiology with Lab ASSIGNMENT: In this unit, you will complete the lab titled Bacterial Isolation. Now it is time to write up your lab report describing the hypothesis and conclusions you drew from the lab experience. By completing the lab report, you will demonstrate your ability to do the following: Describe the lab’s overall objective and make a hypothesis Introduce relevant background knowledge on the topic Summarize the steps...

COURSE   SCIE1046: Fundamental of Microbiology with Lab ASSIGNMENT: In this unit, you will complete the lab titled Identification of Unknown Bacteria: Help save baby Kuppelfangs from an epidemic!. Now it is time to write up your lab report describing the hypothesis and conclusions you drew from the lab experience. By completing the lab report, you will demonstrate your ability to do the following: Describe the lab’s overall objective and make a hypothesis Introduce rele...